Composite
GFP-XylE

Part:BBa_K316006:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-21)

N-terminus his tagged-GFP-XylE fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1106
    Illegal NgoMIV site found at 1278
    Illegal AgeI site found at 1629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

Standard biobrick assembly and PCR primer extension. For full methods please see our wiki[http://2010.igem.org/Team:Imperial_College_London/Strategy]

Source

Existing biobrick parts with modifications using PCR primer extension.